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. 2008 Mar 28;95(1):472–482. doi: 10.1529/biophysj.107.127951

FIGURE 1.

FIGURE 1

The effect of ADP on the principal bend at the switch-point of the beat in bull sperm. All three images show a single frame that corresponds to the time point of greatest curvature development of the principal bend in Triton X-100 extracted cells reactivated to motility with 0.1 mM ATP. The ADP concentration is 0 mM in a, 1 mM in b, and 4 mM in c. All three cells are securely stuck to the slide surface with the flagellum beating freely. In every case, the next frame from the one shown had a reduced principal bend curvature in the region 5–10 μm from the head-tail junction and a portion of the flagellum was reversing the direction of the beat. This indicates that the selected frame is close to the switch-point of beat reversal. Bar = 20 μm.