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ThT fluorescence during the burst of PLA2 activity. Excitation was at λex= 450 nm and λem= 482 nm. (A) 5 mM HEPES, 2 mM CaCl2, 0.1 mM EDTA, pH 7.4 (•). (B) Unbuffered 2 mM CaCl2 (○). Lipid concentration was 200 μM with 6 μM ThT in 2 mM CaCl2. The reactions were started by the addition of PLA2 to a final concentration of 75 nM and were carried out at 40°C.
