FIGURE 6.

(a) The distribution of W_i obtained from the force extension curves recorded at a pulling velocity of 40 nm/s for experiments done at 297 K. (b) Values of ΔG plotted as a function of the reciprocal pulling velocity using the JE for three different temperatures. For experiments at 297 K (squares), the asymptotic behavior of ΔG_JE approaches the adiabatic limit at low pulling velocities. At lower temperatures of 277 K (triangles) and 287 K (circle), the adiabatic limit is not achieved due to the longer lifetimes. The lines connecting the data points can be used as an aid to the eye. Points indicate mean ± SE values.