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. 2008 Mar 13;95(1):333–341. doi: 10.1529/biophysj.107.125328

FIGURE 2.

FIGURE 2

Ice crystals formed in the presence of GFP-sbwAFP. (A) Hexagonal ice crystals with sixfold symmetry decorated with GFP-sbwAFP. Here the c axis is normal to the image plane. (B) Crystals with the c axis in the image plane exhibit fluorescence that appears to have fourfold symmetry. However, this does not represent crystallographic symmetry; the observed structure is merely a projection of a tilted hexagonal crystal (Figs. 2 C and 3). (C) Additional crystals from a separate experiment where the three-dimensional crystal shape is evident. The basal planes, indicated by arrows, are separated by 15 μm. Images were taken with a confocal microscope through a 488 nm filter to visualize GFP.