FIGURE 4.

Cold finger experiment. A single ice crystal was grown from ddH₂O and mounted on a cold finger to seed the growth of a single crystal hemisphere in a solution of GFP-AFPIII (as described by Knight et al. (28)). The c axis was oriented normal to the cold finger and is normal to the figure plane in A and parallel to the figure plane in B. This seed was then immersed in a solution of GFP-AFPIII (see Materials and Methods) and the temperature of the cold finger was gradually lowered to effect the slow growth of the seed into a hemisphere. The hemisphere was then removed from the solution and photographed under UV illumination.