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. 2008 Mar 13;95(1):333–341. doi: 10.1529/biophysj.107.125328

FIGURE 5.

FIGURE 5

Melting and growth of an ice crystal in GFP-sbwAFP solution viewed by epifluorescence microscopy. (A and B) Melting of the ice crystal in the presence of GFP-sbwAFP. (C–E) Accumulation of GFP-sbwAFP on the surface of the ice crystal on cooling within the thermal hysteresis gap. (F and G) Rapid growth of the ice crystal when cooled below the nonequilibrium freezing point. The c axis is marked with a white arrow and is consistent for all the images displayed. Figures experimental time in sec (Movie S1 and Data S1). The temperature in this sequence changes from above the melting temperature (Inline graphic) to below the freezing temperature (Inline graphic).