Figure 1.

Detection of autoantigens in experimental autoimmune uveoretinitis (EAU). A: Shown is a two-dimensional gel image of murine retinal proteins stained with SYPRO Ruby. Approximately 2,000 spots were observed. Among the SYPRO Ruby stained protein spots, the 2D-WB positive spots either in control or EAU were randomly numbered. The numbers and the positions of the seven candidate autoantigens in EAU on a SYPRO Ruby stained gel are shown in panel A. The spot numbers of the candidate autoantigens are common between panel B, Table 1, and Table 2. B: Extracted murine retinal proteins were separated by two-dimensional electrophoresis, transferred onto nitrocellulose membranes. Western blotting was performed using sera from EAU or control mice. Representative membranes reacted with sera from complete Freund's adjuvant-treated control mice are shown in subpanel C, and those reacted with sera from EAU mice are shown in subpanel E. Each set of C and E subpanels shows the corresponding area. Arrowheads indicate the position of each candidate autoantigen on the EAU membranes. membranes.