Table 2. Identification of candidate retinal antigens in experimental autoimmune uveoretinitis.
|
Spot
number |
Protein name |
NCBI accession number |
Calculated MW (kDa)/pI |
MOWSE scorea |
Peptides matched (coverage; %) |
MS/MS sequenceb |
| 103 |
retinol binding protein 3, interstitial |
gi|21729751 |
134/4.98 |
208 |
4 (5%) |
IGQSNFFLTVPVSR |
| 30 |
beta-actin (bAct) |
gi|49868 |
39.4/5.78 |
83 |
1 (4%) |
SYELPDGQVITIGNER |
| 71 |
esterase D (EsteD) |
gi|55777188 |
31.8/6.70 |
52 |
3 (14%) |
SYGQQAASEHGLVVIAPDTSPR |
| 131 |
tubulin beta-2 (Tub-b) |
gi|13542680 |
50.2/4.79 |
144 |
3 (8%) |
FPGQLNADLR |
| 29 |
creatin kinase, brain (BB-CK) |
gi|10946574 |
42.9/5.4 |
209 |
3 (11%) |
VLTPELYAELR |
| 34 |
voltage-dependent anion-selective channel protein |
gi|10720404 |
32.5/8.55 |
39 |
1 (6%) |
WNTDNTLGTEITVEDQLAR |
| 59 | aspartate aminotransferase | gi|871422 | 46.4/6.68 | 142 | 4 (10%) | VGGVQSLGGTGALR |
aProteins with MOWSE score over statistical significant identity (p<0.05) were accepted as identified. bFor the proteins in which several peptides were matched, the peptide identified with the highest MOWSE score is shown.