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Disruption of all 12 immunophilin genes in the dodecuplet mutant yeast strain. Genomic DNA was isolated from the isogenic wild type (JK93da) and the dodecuplet mutant strain (SMY136–1) and analyzed by PCR with primers flanking the immunophilin gene indicated above each lane. (A) PCR analysis of the cyclophilins. (B) PCR analysis of the FKBPs.
