Figure 4.

Transcription of several immunophilins is induced by heat shock and unfolded proteins. Wild type (WT) and mutant (Δ, which is Δcpr1 through Δcpr6, and Δfpr1 through Δfpr3) immunophilin strains were analyzed by Northern blot with probes specific to the gene indicated on the left. Fold induction was determined by normalizing the amounts of immunophilin RNA to actin RNA by PhosphorImaging. The same membrane was used for all blots except for lanes 1–4 of the FPR4 blot. Lanes 1 and 3, untreated (−). Lanes 2 and 4, treated with 10 μg/ml tunicamycin (+) for 3 hr. Lanes 5–9, a wild-type strain (JK93da) was grown at 24°C, heat-shocked at 37°C, and RNA samples were analyzed after 0, 2, 5, 10, and 30 min growth at 37°C.