. Author manuscript; available in PMC: 2008 Jun 12.

Published in final edited form as: J Comp Neurol. 2006 Dec 1;499(4):645–661. doi: 10.1002/cne.21131

TABLE 4.

Numbers and Proportions of Orx+ Cells Contacted by BDA+ Varicosities Positive for Vesicular Transporter Proteins (VTP+), and Numbers and Proportions of BDA+ [VTP+] Varicosities in Contact with Orx+ Cells in the Lateral Hypothalamus (LH)¹

Triple-immunostained BDA/VTP/Orx series	VAChT	VGluT2	VGAT
N° Orx+ cells contacted by BDA+ var. (Orx+:BDA+) in each series	548.3 ± 68.3	532.7 ± 109.7	595.3 ± 109.7
N° Orx+ cells contacted by BDA+ [VTP+] var. (Orx+:BDA+ [VTP+])²	15.7 ± 15.7	188.0 ± 81.4	376.0 ± 27.1
% Orx+ cells contacted by BDA+ [VTP+] var.²	3.7 ± 3.7 %	31.4 ± 9.4 %	66.7 ± 11.2 %
N° BDA+ var. in contact with Orx+ cells (BDA+: Orx+) in each series	908.6 ± 122.4	1096.7 ± 180.7	1457.0 ± 204.9
N° BDA+ [VTP+] var. in contact with an Orx+ cells (BDA+ [VTP+]:Orx+)²	15.7 ± 15.7	203.7 ± 95.3	814.7 ± 87.2
% BDA+ [VTP+] var. in contact with Orx+ cells²	2.2 ± 2.2 %	16.6 ± 6.2 %	57.8 ± 8.7 %

Data are presented as means ± SEM. var., varicosity.

Estimated numbers of cells were obtained by random systematic sampling of the LH area at levels A6.6, A6.2 and A5.8 using StereoInvestigator from three individual cases (injection sites).

According to a non-parametric Kruskal-Wallis test, the different VTP+ varicosities differed significantly according to the numbers (H = 6.587, P = 0.037) and proportions (H = 7.261, P = 0.027) of Orx+ neurons contacted by BDA+ [VTP+] and to the numbers (H = 6.587, P = 0.037) and proportions (H = 6.938, P = 0.031) of BDA+ [VTP+] varicosities contacting Orx+ neurons.