Table 2.
B-42 fusions tested using the Brent two-hybrid system
| LexA fusion | B42 fusion
|
|||||||
|---|---|---|---|---|---|---|---|---|
| alone* | Mβ | Mγ | M5 | M5ΔW | M8 | Mδ | Ato | |
| Mβ | 4 | 71 ± 24 | 64 ± 2 | 75 ± 18 | 93 ± 19 | 42 ± 1 | 92 ± 19 | 17 ± 6 |
| Mγ | 1 | 33 ± 10 | 35 ± 3 | 201 ± 3 | 110 ± 2 | 68 ± 30 | 33 ± 7 | 8 ± 3 |
| M7 | 6 | 50 ± 16 | 29 ± 13 | 213 ± 43 | 71 ± 12 | 55 ± 5 | 31 ± 2 | 28 ± 4 |
| M5 | 0 | 85 ± 44 | 162 ± 94 | 63 ± 34 | 45 ± 25 | 10 ± 6 | 183 ± 115 | 2 ± 1 |
| M5ΔW | 0 | 84 ± 24 | 124 ± 9 | 23 ± 10 | 26 ± 5 | 19 ± 2 | 109 ± 10 | 4 ± 1 |
| M8 | 1 | 88 ± 5 | 125 ± 61 | 20 ± 2 | 18 ± 6 | 10 ± 5 | 22 ± 4 | 4 ± 3 |
| M3 | 0 | 23 ± 9 | 16 ± 8 | 18 ± 2 | 24 ± 2 | 4 ± 2 | 161 ± 73 | 37 ± 12 |
| Mδ | 1 | 78 ± 25 | 25 ± 9 | 69 ± 3 | 45 ± 13 | 16 ± 10 | 37 ± 24 | 5 ± 1 |
| Gro | 0 | 77 ± 3 | 98 ± 17 | 179 ± 66 | 0 | 25 ± 3 | 242 ± 128 | 0 |
The numbers are β-galactosidase units × 10−1. β-galactosidase activity was normalized to the number of cells. Standard deviations are indicated by ±. Above-threshold values are set in bold.
*
Activity tested in the haploid strain bearing only the LexA fusion and the reporter plasmid.