Table 3.
VP16 fusions tested using the Brent two-hybrid system
| LexA fusion | VP16 fusion
|
|||||
|---|---|---|---|---|---|---|
| Ac | Sc | Ase | Da | M3 | Gro | |
| Mβ | 227 ± 88 | 176 ± 53 | 97 ± 23 | 157 ± 40 | 18 ± 3 | 141 ± 6 |
| Mγ | 128 ± 17 | 178 ± 75 | 78 ± 11 | 74 ± 26 | 3 ± 2 | 150 ± 14 |
| M7 | 150 ± 44 | 57 ± 10 | 147 ± 23 | 151 ± 20 | 14 ± 1 | 160 ± 23 |
| M5 | 10 ± 1 | 2 ± 1 | 6 ± 5 | 56 ± 39 | 8 ± 3 | 156 ± 19 |
| M5ΔW | NT | NT | NT | 42 ± 14 | NT | 0 |
| M8 | 10 ± 7 | 1 | 17 ± 11 | 200 ± 36 | 4 ± 1 | 209 ± 51 |
| M3 | 182 ± 51 | 150 ± 28 | 82 ± 22 | 7 ± 4 | 20 ± 4 | 131 ± 19 |
| Mδ | 8 ± 4 | 7 ± 4 | 9 ± 5 | 2 ± 1 | 28 ± 4 | 178 ± 75 |
| Gro | 0 | 1 | 1 | 1 | 28 ± 1 | 10 ± 1 |
The numbers are β-galactosidase units × 10−1. β-galactosidase activity was normalized to the number of cells. Standard deviations are indicated by ±. Above-threshold values are set in bold.
NT: not tested.