Preembedding immunogold labeling in dendritic spines. A, B, Immunogold particles (arrows) within the spine cytoplasm, typically lying away from the membrane. C, D, Particles coding for ARPC-2 were often associated with thin filaments, in the vicinity of branch points (arrows). E, Labeling associated with a likely endosome in a spine profile. F, Gold particles associated with filamentous structures and a coated vesicle (arrow) within a dendritic shaft. Another gold particle is associated with a vesicle lying in the neck of a spine from this shaft. Scale bars, 250 nm.