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. 2008 Jun 18;3(6):e2382. doi: 10.1371/journal.pone.0002382

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cell bodies in the somal compartment were infected with PRV 181, a recombinant expressing an mRFP-VP26 (capsid) fusion and a GFP-VP22 (outer tegument) fusion. Time-lapse images were taken of axons within the microgrooves at 11–14 h postinfection using the Leica SP5 confocal microscope. A typical region of interest is outlined in the brightfield image. Zoom images show an mRFP punctum moving in the retrograde direction (red arrow) and an mRFP punctum co-transporting with GFP-VP22 punctum in anterograde motion (yellow arrow). For a complete time-lapse recording, see Movie S1 in the supporting information. Bar: 50 μm.