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. 2008 Jun 18;3(6):e2382. doi: 10.1371/journal.pone.0002382

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cell bodies in the somal compartment were infected with PRV 181, a recombinant expressing an mRFP-VP26 (capsid) fusion and a GFP-VP22 (outer tegument) fusion. Time-lapse images were taken of axons within the microgrooves at 11–14 h postinfection using the Leica SP5 confocal microscope. Green puncta of light particles containing GFP-VP22 without mRFP-VP26 (green and blue arrows) and yellow punta of heavy particles containing both GFP-VP22 and mRFP-VP26 (yellow and orange arrows) were seen moving in the anterograde direction. Both light and heavy particles displayed heterogeneity in GFP fluorescence. For a complete time-lapse recording, see Movie S2 in the supporting information. Bar: 2 μm.