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. Author manuscript; available in PMC: 2008 Jun 12.
Published in final edited form as: Biochemistry. 2004 May 11;43(18):5138–5148. doi: 10.1021/bi035213q

FIGURE 1.

FIGURE 1

The observed kinetics of 3Dpol-catalyzed nucleotide incorporation in the presence of Mn2+ is dependent upon the nature of the quench. (A) Kinetics of AMP incorporation in the presence of Mn2+ quenched by either EDTA (●) or HCl (○). 2 μM 3Dpol was incubated with 2 μM sym/sub (1 μM duplex) and rapidly mixed with 500 μM ATP (final concentration) in the presence of Mn2+ as described under Experimental Procedures. (B) Kinetics of AMP incorporation in the presence of Mg2+ quenched by either EDTA (●) or HCl (○). 2 μM 3Dpol was incubated with 2 μM sym/sub (1 μM duplex) and rapidly mixed with 500 μM ATP (final concentration) in the presence of Mg2+ as described under Experimental Procedures.