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Elemental effect on the pre-steady-state burst rate of AMP incorporation. (A) 2 μM 3Dpol was incubated with 2 μM sym/sub (1 μM duplex) and rapidly mixed with either 100 μM ATP (●) or 100 μM ATPαS (○) (final concentration) as described under Experimental Procedures. The solid lines represent the fit of the data to a single exponential with a kobs for ATP of 20.4 ± 0.8 s-1 and a kobs for ATPαS of 2.67 ± 0.04 s-1.
