. Author manuscript; available in PMC: 2008 Jun 12.

Published in final edited form as: Biochemistry. 2004 May 11;43(18):5138–5148. doi: 10.1021/bi035213q

Table 3.

Fidelity of 3D^pol-Catalyzed Nucleotide Incorporation in the Presence of Mg²⁺ and Mn²⁺

	fidelity^a
nucleotide	Mg²⁺	Mn²⁺	Mg²⁺/Mn²⁺
2′-dATP	233	13	18
GTP	15477	532	29

Fidelity is calculated as [(k_pol/K_d,app)_ATP + (k_pol/K_d,app)_incorrect]/[(k_pol/K_d,app)_incorrect] (41). sym/sub-U was employed; therefore, ATP is the correct nucleotide. The values for k_pol/K_d,app in the presence of Mg²⁺ were obtained from ref 12, and those in the presence of Mn²⁺ were obtained from Table 1.