Table 5.

Kinetic Parameters for the Kinetic Mechanism of 3D^pol-Catalyzed Nucleotide Incorporation in the Presence of Mn²⁺ (Scheme 1)

parameter	valuea	Keq
k+1	10 μM-1 s-1
k-1	40 s-1	0.25 μM-1
k+2	300 s-1
k-2	100 s-1	3
k+3	30 s-1
k-3	22 s-1	1.4
k+4	≥160 s-1
k-4	<0.0001 s-1	>1.6 × 106
k+5	2000 s-1
k-5	10 μM-1 s-1	200 μM

^aValues for all parameters, with the exception of k_-3 and k_-4, were determined experimentally.