Table 6.

Values for the Rate Constants Employed in Kinetic Simulations Are Constrained by Experimental Data^a

experiment	2-fold change	10-fold change	insensitive
kinetics of AMP incorporation	k+1, k-1,b k+2,c k-2,b k+3	k+2, k+4d	k-3, k-4, k+5, k-5
pulse-chase analysis	k+2, k+3	k+1,d k-2, k-3,e k+4d	k-1, k-4, k+5, k-5
two-nucleotide incorporation (-PPi)	k+1, k+3,c Kd,app,UTP, kpol,UTP	k-1,e k+2,dk-2,e k+3, k-3,e k+4d	k-4, k+5, k-5
two-nucleotide incorporation (+PPi)	k+1, k+3, k+5, k-5, Kd,app,UTP, kpol,UTP	k-1, k+2, k-2,e k-3e	k+4, k-4

^aThe rate constants shown in Table 5 were increased or decreased by 2- or 10-fold, and the fit of the simulation to the indicated experimental data was evaluated. In most instances, either an increase or decrease in the indicated rate constants altered the goodness of fit. However, exceptions were noted, and these are annotated to indicate this fact. Finally, rate constants that can be varied by greater than 10-fold without any apparent change in the fit of the simulation to the data are indicated in the column labeled insensitive.

^bOnly a 2-fold increase gives a significant change in the fit of the simulation to the data.

^cOnly a 2-fold decrease gives a significant change in the fit of the simulation to the data.

^dOnly a 10-fold decrease gives a significant change in the fit of the simulation to the data.

^eOnly a 10-fold increase gives a significant change in the fit of the simulation to the data.