Table 2.

Kinetic Parameters for the Kinetic Mechanism of 3D^pol-Catalyzed Nucleotide Incorporation (Scheme 2)

parameter	valuea	Keq
k+1	10 μM-1s-1
k-1	2140 s-1	4.7 × 10-3 μM-1
k+2	300 s-1
k-2	500 s-1	0.6
k+3	520 s-1
k-3	375 s-1	1.4
k+4	500 s-1
k-4	0.0025 s-1	2.0 × 105
k+5	8100 s-1
k-5	10 μM-1 s-1	810 μM

^aValues for all parameters, with the exception of k_-3, were determined experimentally.