Figure 2. Loss of MMP in Neuro-2a cells correlates with optimum knockdown of Grx1.

Cells were transfected with empty vector (control for 12 and 72 hr; (Fig. 2A)) or shRNA to Grx1 (Fig. 2B) and loss of MMP was monitored after 12, 24, 48 and 72 hr of transfection using JC-1 (2 µg/ml) dye. Loss of MMP in response to downregulation of Grx1 was found to be maximum after 72 hr of transfection. Green staining represents JC-1 monomer in cells with loss of MMP whereas red staining represents JC-1 aggregates in cells with intact MMP. Bar represents 120 µm.