Figure 6. Overexpression of Grx1 prevents loss of MMP and L-BOAA mediated cell toxicity.

Grx1 overexpressing SH-SY5Y clonal cell line and control cell lines electroporated with mock empty vector were characterized for the expression of Grx1 by immunostaining (A), quantitation of mRNA by qRT-PCR (B) and immunoblot (C). SH-SY5Y cells stably overexpressing Grx1 and those electroporated with empty vector (control) were exposed to L-BOAA (1 mM) for 24 hr before determining the cell viability. Cells overexpressing Grx1 show more viability after L-BOAA exposure as compared to the control cells (D). Data is represented as mean±SD from 3 independent experiments. Asterisks indicate values significantly different from controls (p<0.05). SH-SY5Y clonal lines overexpressing Grx1 and mock control cells were subjected to L-BOAA (1 mM) treatment for 24 hr before qualitative determination of their MMP status using JC-1 (2 µg/ml). The former (Grx1 overexpressing cell line) maintained MMP following exposure to L-BOAA while control cells showed loss of MMP detected as green JC-1 monomers (E). Bar represents 120 µm.