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. 2008 Jun 18;3(6):e2459. doi: 10.1371/journal.pone.0002459

Figure 8. Exposure to estrogen or overexpression of Grx1 abolishes L-BOAA induced MMP loss in SH-SY5Y cells.

Figure 8

Cells pretreated with 17-β estradiol (200 nM) and cells stably overexpressing Grx1 were treated with L-BOAA (1 mM; 24 hr). Vehicle treated cells show abrupt decrease in TMRM intensity after CCCP treatment representing sudden loss of MMP (A). Gradual decrease in TMRM intensity in cells treated with L-BOAA represents steady loss of MMP before CCCP treatment, which further decays on adding CCCP (B). MMP is maintained in SH-SY5Y pretreated with 17-β estradiol (C) and in cell lines overexpressing Grx1 (D). L-BOAA mediated loss of MMP is prevented by either pretreating the cells with 17-β estradiol or overexpression of Grx1 (E). Data is represented as mean±SEM from 3 independent experiments. Asterisks indicate values significantly different from controls (p<0.05). Arrow represents time point of addition of CCCP.