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. 2008 Jun 16;181(6):935–944. doi: 10.1083/jcb.200801181

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© 2008 Kressler et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Nsa1-GFP mislocalizes to the cytoplasm in the rix7Δ14N mutant. The subcellular localization of the indicated GFP-tagged proteins was assessed by fluorescence microscopy in wild-type RIX7 and rix7Δ14N mutant cells that were first grown in yeast peptone dextrose at 23°C and then shifted for 3 h to 30°C. Location of Nsa1-GFP was also determined in RIX7 and rix7Δ14N cells that were grown at the permissive temperature of 23°C (top). Bar, 5 μm.