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. 2008 Jun 16;181(6):935–944. doi: 10.1083/jcb.200801181

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© 2008 Kressler et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Nsa1 is associated with translating ribosomes in the rix7Δ14N mutant. Whole cell lysates derived from wild-type RIX7 and rix7Δ14N mutant strains (both expressing Nsa1-TAP), which were first grown at 23°C and then shifted for 3 h to 30°C, were analyzed by sucrose gradient centrifugation under polysome-conserving conditions (A) or polysome runoff conditions (B). The profiles were recorded at A₂₅₄. The peaks of free 40S and 60S subunits, 80S ribosomes, and polysomes are indicated. Half-mers are indicated by arrowheads. Aliquots of the total cell lysate (T) and the fractions from the gradient were analyzed by Western blotting using anti-ProtA and anti-Rpl3 antibodies. The fractions boxed with broken lines indicate the 80S fractions into which the polysomes are redistributed under polysome runoff conditions.