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. 2008 Jun 16;181(6):903–911. doi: 10.1083/jcb.200710151

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© 2008 Winter et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Submitochondrial localization of P1b. (A) Colocalization of P1b–8 EGFP with mitochondria in stably transfected P0 fibroblasts visualized using anti-GFP antibodies and MitoTracker. Bar, 20 μm. (B) Cell lysates and cytosolic and mitochondrial fractions were obtained from fibroblasts stably expressing P1b–8 EGFP, P1–8 EGFP, or EGFP. Mitochondria were extracted with sodium carbonate, and insoluble pellet (Na₂CO₃-P) and soluble supernatant (Na₂CO₃-S) fractions were subjected to immunoblotting. White lines indicate that intervening lanes have been spliced out. (C) Mitochondria were left untreated or lysed with Triton X-100 before the addition of indicated amounts of proteinase K.