Figure 2.

The amino acid sequence encoded by plectin exon 1b serves as a mitochondrion-targeting signal. (A) Fibroblasts expressing the exon 1b–specific sequence, C- or N-terminally fused to EGFP (E1b-EGFP and EGFP-E1b, respectively), were visualized using MitoTracker and anti-GFP antibodies. Bar, 20 μm. (B) Schematic representation of plectin fragments tested and their mitochondrial targeting potential. Plectin's domain structure is shown on top, and actin- (ABD) and IF-binding (IF-BD) domains, the rod domain (ROD), and C-terminal plectin repeat domains 1–6 (black circles) are indicated. (C) Amino acid sequence (residues 1 to 38) encoded by exon 1b. Positively charged residues are depicted in bold letters and negatively charged residues in gray; the transmembrane domain is shaded gray.