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. Author manuscript; available in PMC: 2009 May 6.
Published in final edited form as: Eur J Pharmacol. 2008 Mar 4;585(1):2–13. doi: 10.1016/j.ejphar.2007.11.082

Figure 3. Synapses on spines containing phosphoproteins (pPAK, pCofilin) are larger then their neighbors.

Figure 3

Hippocampal slices received theta burst or control stimulation, were harvested at 7 min, and then tissue was processed for double immunofluorescence for PSD-95 and pCofilin or pPAK. (A) Micrographs show that PSD-95 is colocalized with pCofilin and pPAK in spine-like puncta within CA1 str. radiatum. Micrographs show the individual localization of the phosphoprotein (red, left) and PSD-95 (green, middle) and the overlay of these images (“Merge”, right). In the merged images double labeling appears yellow; arrows indicate individual double-labeled elements. Calibration Bar: 1μm. (B) Plot shows the size frequency distribution of PSD-95 immunoreactive synapses that were associated with pCofilin (“pCof(+)PSDs”, gray line) and those not associated with pCofilin (“pCof(-)PSDs”, black line) in str. radiatum of theta stimulated slices. Note that the size distribution for PSDs associated with pCofilin is shifted toward the right, denoting larger sizes, and has a more normal distribution. A similar rightward shift in the size frequency distribution was found for PSDs associated with pPAK.