TABLE 2.

PROTEOMIC ANALYSIS OF NONTYPEABLE HAEMOPHILUS INFLUENZAE–TREATED LUNG LAVAGE FLUID

	Proteomic Technique
Identified Protein (GenBank Accession No.)	HPLC	iTRAQ	DIGE
Pulmonary surfactant-associated protein D (NP_033186)	•	•	•
Haptoglobin-2 (NP_059066)	•	•	•
Calgranulin B (P31725)	•	•
Kininogen (AAH18158)	•	•
Chitinase-3-like protein 1 (NP_031721)		•	•
Complement C3 (AAH43338)	•		•
Transferrin (NP_598738)	•
Lactoferrin (NP_032548)	•		•
Lysozyme (NP_059068)	•
Alpha-1-protease inhibitor (P22599)	•
Hemopexin (NP_059067)	•
Contrapsin (CAA38948)	•
Vitamin D binding protein (AAA37669)	•
Hemoglobin alpha chain (P01942)		•
Hemoblogin beta chain (P02088)		•
Alpha-1-acid glycoprotein 1 (NP_032794)		•
Inter-alpha-trypsin inhibitor heavy chain H4 (NP_061216)		•
Transketolase (NP_033414)			•
Serpin 1 A protein (NP_079705)			•
Glucose phosphate isomerase (NP_032181)			•
Rho GDI alpha (NP_598557)			•
Polymeric immunoglobulin receptor (NP_035212)			•
Leukotriene E4 hydrolase (NP_032543)			•
Enolase 1 (NP_075608)			•
Lymphocyte cytosolic protein 1 (AAH22943)			•
Lipocalin 2 (NP_032517)			•
WD repeat domain protein 1 (NP_035845)			•

Definition of abbreviations: DIGE = difference gel electrophoresis (see Methods in online supplement for details); iTRAQ = isobaric stable isotope tag.

Proteins listed were found at a higher level in lavage fluid of nontypeable Haemophilus influenzae lysate–treated mice than in untreated mice by one or more of the three proteomic techniques.