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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Surgery. 2007 Aug;142(2):262–269. doi: 10.1016/j.surg.2007.03.011

Figure 3. Representative tissue uptake of rhodamine-labeled p85α siRNA at 24h compared by delivery method.

Figure 3

siRNA uptake, as represented by rhodamine-fluorescence (red), was analyzed 24h following delivery by one of four techniques. Cell nuclei (blue) were counterstained with Hoescht stain. Tissues were collected and processed as previously described. Representative images of the liver, spleen, pancreas, kidney and bone marrow are shown here (magnification = 200x for all images). Rhodamine fluorescence was detected in the liver, spleen, pancreas, kidney and bone marrow following hydrodynamic and standard IV injection. Rhodamine fluorescence was detected in the liver and spleen following intraperitoneal injection. Minimal to no rhodamine fluorescence was detected in control tissues. (IV-H = hydrodynamic IV injection; IV-S = standard IV injection; IP = intraperitoneal administration; PR = rectal administration).

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