Figure 4.

The Size and Distribution of Mapped Protein Domains at Neocentromeres

(A) Innerkinetochore domain organization at seven neocentromeres. The size of the protein-binding domain for each neocentromere is listed. Known protein-coding genes present within these domains are shown (derived from Ensembl release 44). The discontinuous nature of the mardel(10)³⁵ and BBB³⁶ neocentromeres is illustrated. Two possibilities of the layout of the BBB neocentromere are provided—see main text for details. The IMS13q CENPC1 and CENPH domains are marked “? ” to represent an uncertainty as to the size of these domains—the authors of this study suggested that the lower efficiency of ChIP with CENPC1 and CENPH antibodies might prevent the full extent of these domains from being detected.³⁶

(B) Scaffold domains, protein binding domains, and genes present within two mapped neocentromeres. Known protein-coding genes present within these domains are shown (derived from Ensembl release 44), with expression data from cell lines derived from Saffery et al.⁵⁰ and Wong et al.⁶⁷ Differentially expressed genes denote two protein-coding genes found to be activated after neocentromere formation.⁶⁷ (Domain positions are derived from BAC data from Lo et al.,^6,32 Alonso et al.,³³ Cardone et al.,³⁴ and Saffery et al.⁵⁰ updated against Ensembl release 44 from the Ensembl project¹⁰⁵.)