Table 2. Modulation of gene expression in MDM-T cell co-cultures induced by L. chagasi infection.
| MDM-T cell co-cultures: up-regulated transcripts (selected from 116 of 54,675 probe sets†) | ||||
| Symbol | Description | Cytoband | Fold Change | % False Positive |
| CD-69 | CD69 antigen | 12p13-p12 | 1.724 | 0.010 |
| Metallothioneins | ||||
| MT - 1E,1F,1G, 1H,1M,1X, 2A | metallothionein genes | 16q13 | 3.29–37.07 | 0 – 0 |
| Inflammatory and Chemotactic Cytokines | ||||
| IL-2 | interleukin 2 | 4q26-q27 | 4.359 | 0 |
| IFN-γ | interferon, gamma | 12q14 | 2.966 | 0 |
| CXCL-2,-3,-9,-10,-11 | chemokine (C-X-C) ligands 2, 3, 9, 10 and 11 | 4q21, 4q21.2 | 1.62–2.29 | 0.049–0.008 |
| CCL-8, -10 | chemokine (C-C motif) ligands 8, -20 | 17q11.2 | 1.78–2.77 | 0 |
| IL-6 | interleukin 6 | 7p21 | 2.003 | 0.003 |
| TNF-α | tumor necrosis factor, alpha | 6p21.3 | 1.487 | 0.039 |
| IL-1β | interleukin 1, beta | 2q14 | 1.561 | 0.042 |
| IL-1α | interleukin 1, alpha | 2q14 | 1.611 | 0.042 |
| TNFSF-10, TNFSF-11 | tumor necrosis factor (ligand) superfamily, members 10 and 11 | 3q26, 13q14 | 1.69, 1.85 | 0.022, 0.009 |
| Inflammatory Mediators | ||||
| PTGS2 | prostaglandin-endoperox. synthase 2 | 1q25.2-q25.3 | 2.212 | 0.001 |
| STAT1 | signal transducer and activator of transcription 1 | 2q32.2 | 1.860 | 0.006 |
| IFIT2, IFIT3 | interferon-induced protein with tetratricopeptide repeats 2 and 3 | 10q23-q25 | 1.870; 1.786 | 0.008–0.010 |
| CD-36 | CD36 antigen | 7q11.2 | 1.781 | 0.013 |
| C1S | complement component subunit 1s | 12p13 | 1.721 | 0.017 |
| SOD2 | superoxide dismutase 2, mitochondrial | 6q25.3 | 1.672 | 0.031 |
| CSF2 | colony stimulating factor 2 (granulocyte-macrophage) | 5q31.1 | 1.639 | 0.034 |
| Type 2 Transcripts | ||||
| IL-4 | interleukin 4 | 5q31.1 | 1.677 | 0.018 |
| SOCS3 | suppressor of cytokine signaling 3 | 17q25.3 | 1.653 | 0.025 |
| MDM-T cell co-culture: down-regulated transcripts (selected from 19 of 54,675 probe sets†) | ||||
| Symbol | Description | Cytoband | Fold Change | % False Positive |
| Immune Signaling | ||||
| CCR-3 | chemokine (C-C motif) receptor 3 | 3p21.3 | 0.427 | 0 |
| IL1-R2 | interleukin 1 receptor, type II | 2q12-q22 | 0.401 | 0 |
| Cell-Cycle and Amino Acid Metabolism | ||||
| PSAT1 | phosphoserine aminotransferase 1 | 9q21.2 | 0.470 | 0.003 |
| PHGDH | phosphoglycerate dehydrogenase | 1p12 | 0.521 | 0.011 |
| COL6A2 | collagen, type VI, alpha 2 | 21q22.3 | 0.472 | 0.031 |
| COL23A1 | collagen, type XXIII, alpha 1 | 5q35.3 | 0.545 | 0.041 |
| CDK-10 | Cyclin-dependent kinase (CDC2-like) | 16q24 | 0.532 | 0.037 |
| GDF15 | growth differentiation factor 15 | 19p13.1-13.2 | 0.542 | 0.039 |
| MARS2 | methionine-tRNA synthetase 2 | 2q33.1 | 0.543 | 0.047 |
| Collagen subunits | ||||
| COL6A2 | collagen, type VI, alpha 2 | 21q22.3 | 0.472 | 0.031 |
| COL23A1 | collagen, type XXIII, alpha 1 | 5q35.3 | 0.545 | 0.041 |
Genes were selected from the overall list of probe sets found to be differentially regulated by RankProd with a PFP <0.05 and functionally clustered with DAVID.
†: 54,675 probe sets were analyzed, and the indicated number were selected as significantly (PFP<0.05) changed between infected and noninfected macrophages.