Figure 1.

Analysis of deletions of the chi63 promoter region. (A) DNA sequence of the promoter region containing the RNA polymerase binding site (bold letters) and direct repeat sequences (underlined with arrows) and diagrams of deletions of an 850-bp region containing the transcription start site (indicated as +1). (B) Histogram showing the results of quantitative catechol dioxygenase assays plotted as a percentage of the fully induced wild-type level from S. lividans-containing plasmids bearing chi63-xylE transcriptional fusions. pXE60D1, pXE60D2, pXE60D3, pXE60D4, pXE60U1, pXE60U2, and pXE60U3 are pXE60 derivatives that contain different truncated versions of chi63. Promoter-containing plasmids indicated in B refer to the deletions shown in A. Each plasmid-containing strain was assayed after growth on the carbon source indicated.