Figure 7.
Multiple Alignment of the TMEM43 Gene across Eight Eukaryotic and Prokaryotic Species
(A) Clustal W align was used to align orthologs from Homo sapiens (NP_077310), Pan troglodytes (XP_516299), Canis familiaris (XP_541751), Mus musculus (NP_083042), Gallus gallus (XP_414378), Zenopus tropicalis (UP10004D5297), Tetraodon nigroviridis (Q4RXL8), Drosophila melanogaster (NP_64162), and Rhizobium loti (Q98HF3).25 The blue box outlines the DUF1625 domain, and the black boxes outline predicted transmembrane domains. Completely conserved residues are red, strongly similar residues are green, and weakly similar residues are blue. The S358L mutation is marked by a yellow arrow.
(B) The web logo format was used for aligning eukaryotic species. The third transmembrane domain is outlined (black box). The S358L mutation is marked by a yellow arrow.