Figure 8.

Effects of ASOs 09–23 and 10–27 in hSMN2 Transgenic Mice

ASOs 09–23, 10–27, control ASO 00–00, or saline was delivered intravenously via the tail vein, twice a week, at 25 mg/kg. Each ASO or saline was administered to eight mice, and tissues and organs including liver (A), kidney (B), thigh muscles (C) and spinal cord (D) were harvested after 1, 2, 3, or 4 weeks of treatment (two mice each). Total RNA was extracted from tissues with Trizol reagent, and RT-PCR was carried out with a set of hSMN2-specific primers. Radiolabeled PCR products were analyzed by 8% native PAGE and phosphorimaging. The histograms on the right show the corresponding quantitation. Error bars show standard deviations.