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. 2008 Feb 29;82(3):652–660. doi: 10.1016/j.ajhg.2007.12.012

Table 1.

Quantitative RT-PCR Primer and Probe Sequences Used to Amplify and Quantitate the ISCU Transcripts

ISCU Exon Forward Primer (5′–3′) Reverse Primer (5′–3′) Probe
1A/2 CCCCGGCCCGACTCTAT CAGTCCAGTTCCAACATTTTTAGATG AAGGTTGTTGATCATTATGAAAATCCTAGAAACG
1A/1B CCCCGGCCCGACTCTA TTTTGTGTCTCCTCTACAATACTTCACA CACAAGAAGGTATCTCAAA
2/3 GTGGTGACGTAATGAAATTACAGATTC TAATGAGCTGGAGGCAATTGC TGGATGCTAGGTTTAAAACATTTGGCTGTGGTT
3/4 CCACTGAATGGGTGAAAGGAA GCAGAGCTCCTTGGCGATAT CGGTGGAGGAAGCCTTGACTATCAAAAACA
4/5 TGCCTTCCTCCCGTGAAAC CCAGGGCGGCCTTGA ACTGCTCCATGCTGGCTGAAGATGC