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. 2008 May 30;82(6):1241–1248. doi: 10.1016/j.ajhg.2008.04.015

Figure 2.

Figure 2

Hydrolysis of PNPA by CES1 and Its Mutants

Cell S9 fractions prepared from cells transfected with cDNA constructs encoding WT, p.Gly143Glu, and p.Asp260fs CES1 were assayed for their catalytic activity to PNPA hydrolysis. The hydrolytic product PNP was monitored by the absorbance at 405 nm after incubating PNPA with cell S9 (20 μg/ml) at 37°C for 10 min. Data were expressed as the mean ± SD (n = 3).