Figure 6.

Expression of nkx5-3 in Zebrafish and Morpholino Experiments

(A and B) In situ whole-mount 1-day-old-embryo experiments with (A) antisense and (B) sense probes showing polarized expression of nkx5-3 in the eye (arrow) and in the ear (arrowhead). Up and left represent frontal and dorsal parts of animal.

(C and D) Toluidine-blue staining of transverse section in 3 dpf larvae (scale bar represents 0.02 mm). (C) shows a normal embryo, and (D) shows a morpholino-treated embryo from same stage (3 dpf) and with same magnification showing microphthalmia and incomplete retinal stratification.

(E–G) Photographs of the head of zebrafish at 3 dpf. (E) shows a normal embryo, (F) shows a morpholino-treated embryo, and (G) shows a morpholino-treated embryo rescued with nkx5-3 mRNA injection.