Table 1.

Primer Pairs Used to Amplify the BMP4 Gene

		Primer Sequence (5′–3′)
Exon	Annealing Temp (°C)	Forward	Reverse
1	63.5	CTAGGCGAGGTCGGGCGGCTGGAG	CACCTTTTGCCTCGGTCACAGC
2	55	GCTCACGTGACTCCGAGGGGC	CAGGGAGCAGAGTGTGGATATTGTAAAGG
3	50.5	TGCCCCTCCATTTCTAGC	ACTGGGGCTTTGATGTAACC
4a	60	TAGGTTTCCCCTGCATAAGC	GCTTAGGGCTACGCTTGG
4b	55	ATTAGCCGATCGTTACC	GTCCAGCTATAAGGAAGC