Table 1.
Method | Primer Name | Sequence (5′ → 3′) |
---|---|---|
PCR (Amplicon 1) | Promoter-f-A | TGCTACACATACGTTTCTCCATTAC |
Intron1-r | CAAATAGAACTGGTGCTTCTTGT | |
Sequencing (Amplicon 1) | Promoter-f-A | TGCTACACATACGTTTCTCCATTAC |
Promoter-r | CCGGGAAGAAACCTCTGAGT | |
Promoter-f-B | GGTGCAGAGGGACGGCTAAGGAC | |
Intron1-r | CAAATAGAACTGGTGCTTCTTGT | |
PCR (Amplicon 2) | Intron1-f | CCAGCCTCTGAGTGTCCACACA |
Intron5-r | GAGACTCAGGGAACTCCAGA | |
Sequencing (Amplicon 2) | Intron1-f | CCAGCCTCTGAGTGTCCACACA |
Intron3-r | TCCCCATCCCCAGCTGCACGGA | |
Intron3-f | CAGCTGGGGATGGGGATTAGA | |
Intron4-r | GGAGACACCCCTGAGAGCCT | |
Intron4-f | GCTCTGCCCTCAGGAAGGA | |
Intron5-r | GAGACTCAGGGAACTCCAGA | |
PCR (Amplicon 3) | Intron5-f | AAGATCAGCAGTGGTGAGGCA |
Intron7-r-A | TGCTGGCGATGCCGCTGAGTGTGC | |
Sequencing (Amplicon 3) | Intron5-f | AAGATCAGCAGTGGTGAGGCA |
Intron6-r | GACCACCCCCCGCCTCATCCT | |
Intron6-f | AGAAAACAGAGACAGAGGCTCCTA | |
Intron7-r-B | CCCACTGGCTGAGCCCCG | |
PCR (Amplicon 4) | Intron13-f-A | ACAAAATGCAAATAAATGCGCCA |
UTR-r | TGATTTTAGTTTTCTCGGTTAAC | |
Sequencing (Amplicon 4) | Intron13-f-B | AGGACAGACAGATGGACACTGAGA |
UTR-r | TGATTTTAGTTTTCTCGGTTAAC |