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Orai3, but not Orai1, can be activated by 50 μm 2-APB. A-C, averaged [Ca2+]i traces (±S.E.) before and following store depletion by addition of TG in cells transfected with GFP only (A, negative control, n = 12 cells), STIM1 + Orai1 (B, n = 13), and STIM1 + Orai3 (C, n = 11). D-F, averaged [Ca2+]i traces (±S.E.) without store depletion in cells transfected with GFP only (D, n = 8 cells), Orai1 (E, n = 6), and Orai3 (F, n = 8). Solution exchanges are indicated.
