FIGURE 5.

2-APB-induced Orai3 current. A, time course of both inward (-110 mV) and outward (+85 mV) current in a representative control HEK cell overexpressing GFP only. Ca²⁺-free internal solution. B, I-V curves, before (black trace) and after (blue trace) application of 2-APB. Note there is little current under either condition. C, time course of current in a representative HEK cell overexpressing STIM1 and Orai3. D, I-V curves for indicated external solutions. Note the large current with both inward and outward components after 2-APB addition.