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TM23 of Orai3 is responsible for 2-APB sensitization/activation. Averaged [Ca2+]i traces (±S.E.) without Ca2+ store depletion in cells transfected with N-Orai3-Orai1 (A), Orai1-TM-Orai3 (B), Orai1-C-Orai3 (C), Orai1-loop1-Orai3 (D), Orai1-TM12-Orai3 (E), Orai1-TM23-Orai3 (F), Orai1-TM34-Orai3 (G), and Orai1-loop2-Orai3 (H). Solution exchanges are indicated.
