FIGURE 9.
2-APB-induced current from Orai1-Orai3 chimeras. A, time course of both inward (-110 mV) and outward (+85 mV) current in a representative HEK cell overexpressing STIM1 and Orai1-TM-Orai3. Ca2+-free internal solution. B, I-V curves before (black) and after (grey) 2-APB application. Note the black trace represents a relatively large endogenous MIC current. C, time course of current in a representative HEK cell overexpressing STIM1 and Orai1-TM23-Orai3. D, I-V curves for indicated external solutions.