TABLE 1.

Kinetic parameters determined from DNA association and dissociation kinetic data in Mg²⁺ Rate constants are defined in Scheme 1 and obtained from fits shown in supplemental Figs. S2 and S3.

Rate constant	Nucleotide-free state	AMPPNP bound state
kon	109m–1 s–1	109m–1 s–1
koff	120 s–1	20 s–1
k′on	NDa	<1 × 106m–1 s–1
k′off	ND	<1.5 s–1
kiso	ND	0.12 s–1
k–iso	ND	0.035 s–1
k′iso	7 s–1	5.3 s–1
k′–iso	9 s–1	1.5 s–1

^aND means not determined