TABLE 2.
Best fit rate and equilibrium constants to the DNA cleavage data Datasets obtained in different nucleotide states from Figs. 4 and 7 were fit separately to supplemental Scheme 2A.
| Rate or equilibrium constant | Nucleotide-free state | AMPPNP-bound state |
|---|---|---|
| Kclvg,1, obs = Kclvg,1/(1/K′iso + 1) | 0.13 | 0.88 |
| Kclvg,2 | 9.9 | 9.7 |
| k′isoa | >0.1 s–1 (1.2 s–1) | >1 s–1 (1.2 s–1) |
| k′–isoa | >0.1 s–1 (91 s–1) | >0.3 s–1 (12 s–1) |
| kclvg,1a | >0.1 s–1 (5.7 s–1) | >0.3 s–1 (6.4 s–1) |
| k–clvg,1a | >0.3 s–1 (0.58 s–1) | >0.3 s–1 (0.66 s–1) |
| kclvg,2a | 6.6 s–1 (5.7 s–1) | 6.5 s–1 (6.4 s–1) |
| k–clvg,2a | 0.61 s–1 (0.58 s–1) | 0.67 s–1 (0.66 s–1) |
a
Values in parentheses were obtained for a model with the additional constraints, kclvg,1 = kclvg,2 and k–clvg,1 = k–clvg,2