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. 2008 Jun 20;283(25):17158–17167. doi: 10.1074/jbc.M801461200

FIGURE 2.

FIGURE 2.

Northern blot analysis of trp leader RNA in RNase J1 and J2 mutants. A, Northern blot probed with probe 1. Migration of full-length (FL) trp leader RNA and a major RNase J1 cleavage product of ∼100 nt are indicated on the right. Below the gel are the ratios of the intensity of the ∼100-nt RNA relative to FL RNA. RNAs that are larger than trp leader RNA (140 nt) are read-through transcripts, as demonstrated previously (16). Leftmost lanes in parts A and C contain 5′-end-labeled TaqI fragments of plasmid pSE420 DNA (29), and the sizes of these fragments are indicated on the left of the gel. B, high resolution Northern blot analysis of RNA isolated from the wild-type strain, probed with probe 2. Migration of prominent RNA fragments and their sizes (nt) are indicated at the right. Sequencing ladder on the left was generated on M13mp18 single-stranded DNA. C, Northern blot probed with probe 3. The cluster of 3′-terminal fragments is indicated on the right as running between 30–40 nt. Below the gel are the amounts, relative to the amount in wild type, of these fragments. D, high resolution Northern blot analysis of RNA isolated from the wild-type strain, probed with probe 3. Migration of prominent RNA fragments and their sizes (nt) are indicated at the right. WT, wild type.