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Analysis of reqIgG7 mutants by SDS-PAGE and Western blotting. Proteins were run under non-reducing (A) and reducing (B and C) conditions and stained with Coomassie Brilliant blue (A and B) or immunoblotted and probed with polyclonal goat anti-horse IgGb which recognizes reqIgG4 and reqIgG7 (C). Lane 1, wild-type reqIgG7; lane 2, M252K; lane 3, K382Q; lane 4, H433R; lane 5, HN433/434EH; lane 6, HY435/436TV.
